Deleted
Sample Discontinued
Experience: None
Not Used
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Composite

Part:BBa_K136040:Design

Designed by: alexandra bouaziz   Group: iGEM08_Paris   (2008-10-25)


pFlgA-RFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3137
    Illegal XbaI site found at 3152
    Illegal SpeI site found at 212
    Illegal PstI site found at 1095
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3137
    Illegal SpeI site found at 212
    Illegal PstI site found at 1095
    Illegal NotI site found at 1088
    Illegal NotI site found at 3143
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3137
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3137
    Illegal XbaI site found at 3152
    Illegal SpeI site found at 212
    Illegal PstI site found at 1095
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3137
    Illegal XbaI site found at 3152
    Illegal SpeI site found at 212
    Illegal PstI site found at 1095
    Illegal AgeI site found at 791
    Illegal AgeI site found at 903
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2176


Design Notes

pFlgA was digested by EcoRI ,SpeI (FI )and cloned into the J61002 plasmid containing RFP (BV).


Source

flgA promotor comes from E. coli K12 strain MG1655.

References